Making and Genetically Manipulating Mouse Embryos

Location: The Jackson Laboratory in Bar Harbor, Maine

Are you looking to enhance your research capabilities with advanced techniques in mouse embryo generation and genetic manipulation? Join us for Making and Genetically Manipulating Mouse Embryos, a hands-on workshop providing foundational knowledge and technical skills training for innovative mouse model development.

In-Person Capacity: 16

Mar

10 - 13

2025

About

This immersive workshop spans critical aspects of mouse embryo generation and genetic manipulation for new model development.

Hands-on Sessions Include:

How to generate mouse embryos via in-vitro fertilization and natural mating with oviduct flushing
CRISPR reagent delivery for novel mouse model development via electroporation of zygotes
Surgical and non-surgical (NSET) embryo transfer
Embryo cryopreservation and recovery (controlled rate and vitrification methods)
Microcapillary transfer pipetting
Embryo evaluation
Vasectomy surgery
Selecting mice in estrus
Male and female reproductive anatomy

Didactic Sessions Include:

CRISPR-based mouse model development
How to generate pseudopregnant females
IVF (strain selection, superovulation, project planning and trouble shooting)
Fundamentals of cryobiology
Mouse reproductive anatomy
Aseptic surgical techniques and biomethods

Why Attend?

This in-person workshop offers a unique opportunity to develop your skills in a supportive and interactive environment, learning from experts at JAX. By the end, you will have the information and foundational skills necessary to implement these advanced techniques at your home institution.

Completion of the workshop may be used towards continuing education units for AALAS and Academy of Surgical Research (ASR) re-certification.

Accommodation Information

We strive to host inclusive, accessible events that enable all individuals, including individuals with disabilities, to engage fully. To request accommodation or for inquiries about accessibility, please contact Erin McDevitt at 207-288-6659 or [email protected].

Registration

Waitlist

Registration for this workshop is at capacity. Sign up to be added to the waitlist and to be notified when this course opens again next year. This list will be contacted if space becomes available for this course. Contact the event planner for more information.

Waitlist Open

Travel Information

Attendees will be responsible for their own travel and lodging arrangements for the duration of this event.

On-Campus

Lodging accommodations at JAX’s Highseas Conference Center is available for an additional fee. Highseas, located approximately 1 mile from The Jackson Laboratory, is a spacious estate built in 1912, overlooking the entrance to Frenchman Bay and adjacent to beautiful Acadia National Park. Attendees are responsible for their own transportation to the Conference Center. We do provide shuttles to and from the main campus for the duration of the course. Highseas is handicap accessible (please indicate accessibility needs in registration). This lodging option cannot accommodate families. Rooms may be shared with other participants.

Off-Campus

Participants interested in securing off-campus lodging may refer to the Bar Harbor Chamber of Commerce website for a guide on hotels, motels, bed & breakfasts, and short-term rentals in the Bar Harbor area.

Meals will be provided by JAX’s campus dining services throughout the event. Participants staying off-campus may still partake in evening meals at Highseas. Please refer to the event schedule for more information.

Participants must adhere to Maine State travel, health and safety guidelines at the time of the event and use good judgement when arranging lodging. We recommend participants be familiar with their hotel’s cancellation policies prior to booking. The Jackson Laboratory is not responsible for lodging expenses or losses incurred due to the cancellation or rescheduling of an event.