C57BL/6J-Tg(Nkx2-1-cre)2Sand/J

Stock Number: 008661

C57BL/6J-Tg(Nkx2-1-cre)2Sand/J males were mated to female Rosa 26 reporters (stock no 3474).  Reporter (β-galactosidase) expression is expected in brain interneuron progenitors, developing lung, thyroid, and pituitary by the Nkx2.1 promoter/enhancer regions. Primary Reference.

β-galactosidase expression was assayed for E10.5 and E15.5 whole embryos, and P7 and Adult adipose, brain, bone, epididymis, eye, heart, intestine, kidney, liver, lung, lymph node, muscle, ovary, pancreas, skin, spinal cord, spleen, testes, thymus, and uterus.

C57BL/6J-Tg(Nkx2-1-cre)2Sand/J recombinase activity data also available in MGI Creportal.

Age and Tissue Reporter Expression Image

Embryonic Day 10.5
---Embryo

β-gal expression is observed in:
Developing Brain - restricted in floor of diencephalon, early pituitary/infindibulum, ventral hindbrain, and dorsal telencephalon
Cephalic Mesenchyme - restricted
Thyroid Primordium - restricted
Lung Bud - limited/ restricted
Rathke's Pouch - restricted

Nkx2-cre E10.5 Nkx2-cre E10.5

Nkx2-cre E10.5

Embryonic Day 15.5
---Embryo

β-gal expression is observed in:
Developing Brain - restricted in hypothalamus and forebrain
Thyroid - restricted
Lung - restricted in developing airways
Nkx2-cre E15.5 Nkx2-cre E15.5
Nkx2-cre E15.5 

Postnatal Day 7
---Brain

β-gal expression is observed in:
Cerebral Cortex - scattered cells
Hippocampus - scattered cells
Hypothalamus - strong and restricted
Nkx2-cre P7 Brain

Postnatal Day 7
---Lung

β-gal expression is observed in:
Bronchus - restricted
Alveolus/Parenchyme - widespread
Nkx2-cre P7 Lung

Postnatal Day 7
---Adipose
---Bone
---Epididymis
---Eye
---Heart
---Intestine
---Kidney
---Liver
---Lymph Node
---Muscle
---Ovary
---Pancreas
---Skin
---Spinal Cord
---Spleen
---Testes
---Thymus
---Uterus

Absent Images are not shown for negative tissues.

Adult
---Brain

β-gal expression is observed in:
Cerebral Cortex - scattered cells
Hippocampus - scattered cells
Caudoputamen - scattered cells
Hypothalamus - strong and restricted

Nkx2-cre Adult Brain

Nkx2-cre Adult Brain

Adult
---Lung
β-gal expression is observed in:
Bronchus - restricted
Alveolus/Parenchyme - homogenous
Nkx2-cre Adult Lung Nkx2-cre Adult Lung

Adult
---Adipose
---Bone
---Epididymis
---Eye
---Heart
---Intestine
---Kidney
---Liver
---Lymph Node
---Muscle
---Ovary
---Pancreas
---Skin
---Spinal Cord
---Spleen
---Testes
---Thymus
---Uterus

Absent

Images are not shown for negative tissues.

 

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